Baicalein Triggers Autophagy and Inhibits the Protein Kinase B/Mammalian Target of Rapamycin Pathway in Hepatocellular Carcinoma HepG2 Cells
Identifieur interne : 000F76 ( Main/Exploration ); précédent : 000F75; suivant : 000F77Baicalein Triggers Autophagy and Inhibits the Protein Kinase B/Mammalian Target of Rapamycin Pathway in Hepatocellular Carcinoma HepG2 Cells
Auteurs : Ya-Fang Wang [République populaire de Chine] ; Ting Li [République populaire de Chine] ; Zheng-Hai Tang [République populaire de Chine] ; Lin-Lin Chang [République populaire de Chine] ; Hong Zhu [République populaire de Chine] ; Xiu-Ping Chen [République populaire de Chine] ; Yi-Tao Wang [République populaire de Chine] ; Jin-Jian Lu [République populaire de Chine]Source :
- Phytotherapy Research [ 0951-418X ] ; 2015-05.
Abstract
Baicalein (BA), isolated from the Chinese medicinal herb Scutellariae radix (Huangqin in Chinese), is a flavonoid with various pharmacological effects. Herein, we found that BA only slightly reduced the cell viability on HepG2 cells after 24‐h treatment as determined by 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyl tetrazolium bromide (MTT) assay. However, BA (50 μM) effectively blocked the colony formation. Meanwhile, BA remarkably induced the formation of autophagosomes after 24‐h treatment as determined by immunofluorescence with monodansylcadaverine staining as well as transmission electron microscopy, respectively. Moreover, BA obviously up‐regulated the expression of microtubule‐associated protein 1A/1B‐light chain 3‐II in concentration‐dependent and time‐dependent manners in HepG2 cells. When combined with the autophagy inhibitor chloroquine and BA, the cell viability and colony formation were significantly decreased, indicating that BA triggered protective autophagy, which prevented cell death. Further study showed that BA concentration‐dependently and time‐dependently decreased the expression of p‐AKT (S473), p‐ULK1 (S757) and p‐4EBP1 (T37 and S65), suggesting the involvement of protein kinase B (AKT)/mammalian target of rapamycin (mTOR) in BA‐triggered autophagy. Copyright © 2015 John Wiley & Sons, Ltd.
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DOI: 10.1002/ptr.5298
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Herein, we found that BA only slightly reduced the cell viability on HepG2 cells after 24‐h treatment as determined by 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyl tetrazolium bromide (MTT) assay. However, BA (50 μM) effectively blocked the colony formation. Meanwhile, BA remarkably induced the formation of autophagosomes after 24‐h treatment as determined by immunofluorescence with monodansylcadaverine staining as well as transmission electron microscopy, respectively. Moreover, BA obviously up‐regulated the expression of microtubule‐associated protein 1A/1B‐light chain 3‐II in concentration‐dependent and time‐dependent manners in HepG2 cells. When combined with the autophagy inhibitor chloroquine and BA, the cell viability and colony formation were significantly decreased, indicating that BA triggered protective autophagy, which prevented cell death. Further study showed that BA concentration‐dependently and time‐dependently decreased the expression of p‐AKT (S473), p‐ULK1 (S757) and p‐4EBP1 (T37 and S65), suggesting the involvement of protein kinase B (AKT)/mammalian target of rapamycin (mTOR) in BA‐triggered autophagy. Copyright © 2015 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Zhejiang</li></region><settlement><li>Hangzhou</li></settlement><orgName><li>Université de Zhejiang</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Ya Ang" sort="Wang, Ya Ang" uniqKey="Wang Y" first="Ya-Fang" last="Wang">Ya-Fang Wang</name></noRegion><name sortKey="Chang, Lin In" sort="Chang, Lin In" uniqKey="Chang L" first="Lin-Lin" last="Chang">Lin-Lin Chang</name><name sortKey="Chen, Xiu Ing" sort="Chen, Xiu Ing" uniqKey="Chen X" first="Xiu-Ping" last="Chen">Xiu-Ping Chen</name><name sortKey="Li, Ting" sort="Li, Ting" uniqKey="Li T" first="Ting" last="Li">Ting Li</name><name sortKey="Lu, Jin Ian" sort="Lu, Jin Ian" uniqKey="Lu J" first="Jin-Jian" last="Lu">Jin-Jian Lu</name><name sortKey="Tang, Zheng Ai" sort="Tang, Zheng Ai" uniqKey="Tang Z" first="Zheng-Hai" last="Tang">Zheng-Hai Tang</name><name sortKey="Wang, Yi Ao" sort="Wang, Yi Ao" uniqKey="Wang Y" first="Yi-Tao" last="Wang">Yi-Tao Wang</name><name sortKey="Zhu, Hong" sort="Zhu, Hong" uniqKey="Zhu H" first="Hong" last="Zhu">Hong Zhu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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